Scheme 1.

Overview of the workflow in this study. First, 100 or 1000 sorted THP‐1 cells were collected in a BSA‐coated tube or a hydrophilic‐coated tube, and following each sample processing procedure, protein profiling was performed. Five Groups were prepared: Group 1: 1000 cells in a BSA‐coated tube digested with medium; Group 2: 1000 cells in a BSA‐coated tube with the medium removed; Group 3: 1000 cells in a hydrophilic‐coated tube with the medium removed; Group 4: 100 cells in a BSA‐coated tube digested with medium; Group 5: 100 cells in a BSA‐coated tube with the medium removed. Schematic diagram of cell sorting in the microfluidic‐based cell sorter and 1000‐sorted cells gated by HLA‐DR⁺. The original diagram was kindly provided by On‐chip Biotechnologies Co., Ltd.