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. 2017 Jul 4;47(8):1342–1353. doi: 10.1002/eji.201646765

Figure 3.

Figure 3

Impaired pro‐inflammatory cytokine expression in Card9‐deficient lamina propria leukocytes during colitis. Acute colitis was induced in WT and Card9−/− mice by feeding with DSS for 7 days, followed by 5 days of recovery. (A) Relative expression of Trefoil factor 3 (TFF3), Keratinocyte growth factor (KGF), and IL‐18 mRNA was measured by quantitative PCR and normalized to β‐actin transcript levels in colon tissues of untreated WT (n = 5) and Card9−/− (n = 5), or acute DSS‐treated WT (n = 4) and Card9−/− (n = 4) mice at day 12 of the experiment (DSS). (B) IL‐1β, IL‐17A, IL‐22, and TNF transcript levels in lamina propria leucocytes of untreated WT (n = 5) and Card9−/− (n = 5), or acute DSS‐treated WT (n = 4) and Card9−/− (n = 5) mice at day 12 of the experiment (DSS) were analyzed by qPCR as described above. The data of one representative experiment are shown as the mean + S.E.M of the indicated numbers of samples. * p < 0.05, Student's t‐test; n.s., not significant.