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Comparison of RL production in liquid cultures between WT and isolated mutant strains quantified using LC–MS/MS in multiple reaction monitoring mode. Error bars indicate the standard deviations of biological triplicates. Significant differences were determined between WT and mutants using an independent two-tailed, two-sample t-test for equal sample sizes and equal variance. Significance levels: * p < 0.05; ** p < 0.01; *** p < 0.001.
