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. Author manuscript; available in PMC: 2017 Sep 15.
Published in final edited form as: Cell Rep. 2016 Feb 11;14(7):1662–1672. doi: 10.1016/j.celrep.2016.01.030

Figure 7. Luminal Cardiomyocyte Division Angles in E8.5 NuMA Cardiac Null Mouse Ventricles.

Figure 7

A. Top panels: Representative images of early mitotic luminal cardiomyocytes in E8.5 NuMA cardiac null hearts co-stained for pHH3 (green), Pcnt (yellow), TnT (purple), and DAPI (blue). Lower panels: Superimposed axis of cell division (solid line) relative to the cardiac lumen (dashed line). B. Radial histogram depicting the orientation of cardiomyocyte divisions in early-stage mitotic cells from E8.5 NuMA cardiac null hearts. C. Top panels: Representative images of luminal telophase cardiomyocytes in E8.5 NuMA cardiac null hearts co-stained for survivin (green), TnT (red), and DAPI (blue). Lower panels: Superimposed axis of cell division (solid line) relative to the cardiac lumen (dashed line). D. Radial histogram depicting the orientation of cardiomyocyte divisions in late-stage mitotic cells from E8.5 NuMA cKO hearts. Scale bars: 10µm. P values (P≤0.01 for early and P≤0.001 for late) determined by a chi-square test in B&D for determining significance of the difference between cardiac null and wildtype cells. Chi-square P values related to B&D are in Table S1. n values in B&D represent the number of cells analyzed from 3 to 5 independent animals.