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. Author manuscript; available in PMC: 2018 Sep 7.
Published in final edited form as: Cell Stem Cell. 2017 Aug 17;21(3):349–358.e6. doi: 10.1016/j.stem.2017.07.014

KEY RESOURCES TABLE.

REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Rabbit anti-Pax6 BioLegend PRB-278P
RRID: AB_2313780
Mouse anti-Pax6 BD Bioscience 561664
RRID: AB_10895587
Rabbit anti-Tbr2 Abcam ab23345
RRID: AB_778267
Chicken anti-GFP Aveslab GFP-1020
RRID: AB_10000240
Mouse anti-Ki67 BD Bioscience 550609
RRID: AB_393778
Goat anti-Sox2 Santa Cruz sc-17320
RRID: AB_2286684
Chicken anti-Nestin Aveslab NES
RRID: AB_2314882
Mouse anti-PKCλ BD Bioscience 610207
RRID: AB_397606
Mouse anti-N-Cadherin Innovative Research 18-0224
RRID: AB_86735
Rabbit anti-Actin Cytoskeleton AAN01
RRID: AB_10708070
Mouse anti-β-Catenin BD Bioscience 610153
RRID:AB_397554
Rabbit anti-ZO-1 Thermo Fisher Scientific 40-2200
RRID:AB_10104693
Rabbit anti- Numbl Abcam ab37500
RRID:AB_881766
Rabbit anti-SMAD7 Thermo Fisher Scientific 42-0400 RRID:AB_2533512
Rabbit anti-ZIKV NS1 GeneTex GTX133307
Goat anti-GFP Rockland 600-101-215 RRID:AB_218182
Rabbit anti-Calnexin Abcam ab10286 RRID:AB_2069009
Human serum of ZIKV-infected patients (Li et al., 2016) N/A
Chemicals, Peptides, and Recombinant Proteins
ProLong Gold Antifade Mountant Thermo Fisher Scientific P10144
DAPI Thermo Fisher Scientific D1306
RRID: AB_2629482
StemPro Accutase Cell Dissociation Reagent Thermo Fisher Scientific A1110501
Matrigel Matrix Corning 354277
SimplyBlue SafeStain Thermo Fisher Scientific LC6060
Y-27632 Cellagen Technology C9127-2s
EdU Thermo Fisher Scientific A10044
Phosphatase Inhibitor Cocktail Cell Signaling 5870
Protease Inhibitor Cocktail Sigma P8340
4× Laemmli Sample Buffer Bio-Rad 1610747
A83-01 Stemcell Technologies 72022
Dorsomorphin Stemcell Technologies 72102
SB431542 Stemcell Technologies 72232
CHIR99021 Stemcell Technologies 72052
Dulbecco’s Phosphate-Buffered Saline (DPBS) Corning 21-031
Dulbecco’s Modification of Eagle’s Medium (DMEM) Corning 10-013
DMEM/F-12, HEPES Gibco 11330-032
Neurobasal Medium Gibco 21103049
KnockOut Serum Replacement Gibco 10828028
GlutaMAX Supplement Gibco 35050061
MEM Non-Essential Amino Acids Solution Gibco 11140050
Penicillin-Streptomycin (10,000 U/mL) Gibco 15140122
2-Mercaptoethanol Gibco 21985023
N-2 Supplement Gibco 17502048
B-27 Supplement Gibco 17504044
Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix Corning 354230
Insulin solution Sigma-Aldrich I0516
Fetal Bovine Serum (FBS) Corning 35-010
0.1% Gelatin in Water Stemcell Technologies 7903
Advanced DMEM/F-12 Gibco 12634010
Human recombinant LIF Stemcell Technologies 78055
Compound E Stemcell Technologies 73952
MG-132 Cell Signaling 2194
Recombinant Human FGF-basic Peprotech 100-18B
Recombinant Human EGF-basic R&D Systems P01133
Polybrene Infection/Transfection Reagent Thermo Fisher Scientific TR1003G
Bafilomycin A1 Sigma-Aldrich B1793
Delanzomib Kindly gifted by Dr. Wei Zheng (NIH/NCATS) N/A
Critical Commercial Assays
Click-iT EdU Assay Kits Thermo Fisher Scientific C10340
Cy5-NHS ester GE Healthcare PA15101
SuperScript III First-Strand Synthesis System Thermo Fisher Scientific 18080051
Fast SYBR Green Master Mix Thermo Fisher Scientific 4385610
Lipofectamine 2000 Thermo Fisher Scientific 11668027
Anti-HA Magnetic Beads Thermo Fisher Scientific 88836
Endoplasmic Reticulum Isolation Kit Sigma-Aldrich ER0100
RNeasy Mini Kit Qiagen 74104
Experimental Models: Cell Lines
HEK293 ATCC CRL-1573, RRID:CVCL_0045
C12 (iPSC from normal human foreskin fibroblasts) (Wen et al., 2014) N/A
Experimental Models: Organisms/Strains
Mouse: Crl:CD1(ICR) Charles River Laboratory RRID: IMSR_CRL:22
Mouse: Crl:CF188 Charles River Laboratory RRID:IMSR_CRL:23
Yeast: Y258 Kindly gifted by Dr. Michael Snyder (Yale University) N/A
Virus: ZIKV-MR766 ZeptoMetrix 0810521CF
Virus: ZIKV-SZ (Li et al., 2016) N/A
Oligonucleotides
Primers for ZIKV ORF cloning See Table S1 N/A
Primers for Q-PCR analysis See Table S1 N/A
Recombinant DNA
pDONR221 Thermo Fisher Scientific 12536017
pCAG-GFP (Matsuda and Cepko, 2004) Addgene plasmid: 11150
pEGH-A (Zhu et al., 2009) N/A
pCWX-R4-DEST-R2-PG (Giry-Laterriere et al., 2011) Addgene plasmid: 45957
pENTR-L4-Ubi-L1R (Giry-Laterriere et al., 2011) Addgene plasmid: 45959
Software and Algorithms
Image J NIH https://imagej.nih.gov/ij/
Imaris Bitplane http://www.bitplane.com/imaris/imaris
MATLAB MathWorks https://www.mathworks.com/products/matlab.html
STRING 10.0 database (Szklarczyk et al., 2015) http://string-db.org/
DAVID 6.8 (Huang da et al., 2009) https://david.ncifcrf.gov/
Other
SuperSignal West Dura Extended Duration Substrate Thermo Fisher Scientific 34075
Costar 6 Well Clear Flat Bottom Ultra Low Attachment plate Sigma-Aldrich CLS3471
Nucleofector Kits for Mouse Neural Stem Cells Lonza VAPG-1004
Nucleofector 2b Device Lonza AAB-1001
Square wave electroporator Nepa Gene CUY21SC
Tweezers with platinum disk electrode Nepa Gene CUY650-5
Immun-Blot PVDF Membrane Bio-Rad 1620177
12-well Spinning Bioreactor (Qian et al., 2016) N/A
LSM 800 confocal microscope Zeiss LSM 800
Leica CM3050S Research Cryostat Leica Biosystems CM3050S