KEY RESOURCES TABLE.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-Pax6 | BioLegend | PRB-278P RRID: AB_2313780 |
| Mouse anti-Pax6 | BD Bioscience | 561664 RRID: AB_10895587 |
| Rabbit anti-Tbr2 | Abcam | ab23345 RRID: AB_778267 |
| Chicken anti-GFP | Aveslab | GFP-1020 RRID: AB_10000240 |
| Mouse anti-Ki67 | BD Bioscience | 550609 RRID: AB_393778 |
| Goat anti-Sox2 | Santa Cruz | sc-17320 RRID: AB_2286684 |
| Chicken anti-Nestin | Aveslab | NES RRID: AB_2314882 |
| Mouse anti-PKCλ | BD Bioscience | 610207 RRID: AB_397606 |
| Mouse anti-N-Cadherin | Innovative Research | 18-0224 RRID: AB_86735 |
| Rabbit anti-Actin | Cytoskeleton | AAN01 RRID: AB_10708070 |
| Mouse anti-β-Catenin | BD Bioscience | 610153 RRID:AB_397554 |
| Rabbit anti-ZO-1 | Thermo Fisher Scientific | 40-2200 RRID:AB_10104693 |
| Rabbit anti- Numbl | Abcam | ab37500 RRID:AB_881766 |
| Rabbit anti-SMAD7 | Thermo Fisher Scientific | 42-0400 RRID:AB_2533512 |
| Rabbit anti-ZIKV NS1 | GeneTex | GTX133307 |
| Goat anti-GFP | Rockland | 600-101-215 RRID:AB_218182 |
| Rabbit anti-Calnexin | Abcam | ab10286 RRID:AB_2069009 |
| Human serum of ZIKV-infected patients | (Li et al., 2016) | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| ProLong Gold Antifade Mountant | Thermo Fisher Scientific | P10144 |
| DAPI | Thermo Fisher Scientific | D1306 RRID: AB_2629482 |
| StemPro Accutase Cell Dissociation Reagent | Thermo Fisher Scientific | A1110501 |
| Matrigel Matrix | Corning | 354277 |
| SimplyBlue SafeStain | Thermo Fisher Scientific | LC6060 |
| Y-27632 | Cellagen Technology | C9127-2s |
| EdU | Thermo Fisher Scientific | A10044 |
| Phosphatase Inhibitor Cocktail | Cell Signaling | 5870 |
| Protease Inhibitor Cocktail | Sigma | P8340 |
| 4× Laemmli Sample Buffer | Bio-Rad | 1610747 |
| A83-01 | Stemcell Technologies | 72022 |
| Dorsomorphin | Stemcell Technologies | 72102 |
| SB431542 | Stemcell Technologies | 72232 |
| CHIR99021 | Stemcell Technologies | 72052 |
| Dulbecco’s Phosphate-Buffered Saline (DPBS) | Corning | 21-031 |
| Dulbecco’s Modification of Eagle’s Medium (DMEM) | Corning | 10-013 |
| DMEM/F-12, HEPES | Gibco | 11330-032 |
| Neurobasal Medium | Gibco | 21103049 |
| KnockOut Serum Replacement | Gibco | 10828028 |
| GlutaMAX Supplement | Gibco | 35050061 |
| MEM Non-Essential Amino Acids Solution | Gibco | 11140050 |
| Penicillin-Streptomycin (10,000 U/mL) | Gibco | 15140122 |
| 2-Mercaptoethanol | Gibco | 21985023 |
| N-2 Supplement | Gibco | 17502048 |
| B-27 Supplement | Gibco | 17504044 |
| Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix | Corning | 354230 |
| Insulin solution | Sigma-Aldrich | I0516 |
| Fetal Bovine Serum (FBS) | Corning | 35-010 |
| 0.1% Gelatin in Water | Stemcell Technologies | 7903 |
| Advanced DMEM/F-12 | Gibco | 12634010 |
| Human recombinant LIF | Stemcell Technologies | 78055 |
| Compound E | Stemcell Technologies | 73952 |
| MG-132 | Cell Signaling | 2194 |
| Recombinant Human FGF-basic | Peprotech | 100-18B |
| Recombinant Human EGF-basic | R&D Systems | P01133 |
| Polybrene Infection/Transfection Reagent | Thermo Fisher Scientific | TR1003G |
| Bafilomycin A1 | Sigma-Aldrich | B1793 |
| Delanzomib | Kindly gifted by Dr. Wei Zheng (NIH/NCATS) | N/A |
| Critical Commercial Assays | ||
| Click-iT EdU Assay Kits | Thermo Fisher Scientific | C10340 |
| Cy5-NHS ester | GE Healthcare | PA15101 |
| SuperScript III First-Strand Synthesis System | Thermo Fisher Scientific | 18080051 |
| Fast SYBR Green Master Mix | Thermo Fisher Scientific | 4385610 |
| Lipofectamine 2000 | Thermo Fisher Scientific | 11668027 |
| Anti-HA Magnetic Beads | Thermo Fisher Scientific | 88836 |
| Endoplasmic Reticulum Isolation Kit | Sigma-Aldrich | ER0100 |
| RNeasy Mini Kit | Qiagen | 74104 |
| Experimental Models: Cell Lines | ||
| HEK293 | ATCC | CRL-1573, RRID:CVCL_0045 |
| C12 (iPSC from normal human foreskin fibroblasts) | (Wen et al., 2014) | N/A |
| Experimental Models: Organisms/Strains | ||
| Mouse: Crl:CD1(ICR) | Charles River Laboratory | RRID: IMSR_CRL:22 |
| Mouse: Crl:CF188 | Charles River Laboratory | RRID:IMSR_CRL:23 |
| Yeast: Y258 | Kindly gifted by Dr. Michael Snyder (Yale University) | N/A |
| Virus: ZIKV-MR766 | ZeptoMetrix | 0810521CF |
| Virus: ZIKV-SZ | (Li et al., 2016) | N/A |
| Oligonucleotides | ||
| Primers for ZIKV ORF cloning | See Table S1 | N/A |
| Primers for Q-PCR analysis | See Table S1 | N/A |
| Recombinant DNA | ||
| pDONR221 | Thermo Fisher Scientific | 12536017 |
| pCAG-GFP | (Matsuda and Cepko, 2004) | Addgene plasmid: 11150 |
| pEGH-A | (Zhu et al., 2009) | N/A |
| pCWX-R4-DEST-R2-PG | (Giry-Laterriere et al., 2011) | Addgene plasmid: 45957 |
| pENTR-L4-Ubi-L1R | (Giry-Laterriere et al., 2011) | Addgene plasmid: 45959 |
| Software and Algorithms | ||
| Image J | NIH | https://imagej.nih.gov/ij/ |
| Imaris | Bitplane | http://www.bitplane.com/imaris/imaris |
| MATLAB | MathWorks | https://www.mathworks.com/products/matlab.html |
| STRING 10.0 database | (Szklarczyk et al., 2015) | http://string-db.org/ |
| DAVID 6.8 | (Huang da et al., 2009) | https://david.ncifcrf.gov/ |
| Other | ||
| SuperSignal West Dura Extended Duration Substrate | Thermo Fisher Scientific | 34075 |
| Costar 6 Well Clear Flat Bottom Ultra Low Attachment plate | Sigma-Aldrich | CLS3471 |
| Nucleofector Kits for Mouse Neural Stem Cells | Lonza | VAPG-1004 |
| Nucleofector 2b Device | Lonza | AAB-1001 |
| Square wave electroporator | Nepa Gene | CUY21SC |
| Tweezers with platinum disk electrode | Nepa Gene | CUY650-5 |
| Immun-Blot PVDF Membrane | Bio-Rad | 1620177 |
| 12-well Spinning Bioreactor | (Qian et al., 2016) | N/A |
| LSM 800 confocal microscope | Zeiss | LSM 800 |
| Leica CM3050S Research Cryostat | Leica Biosystems | CM3050S |