Video 1. Typical withdrawal response in rats.

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This video clip shows two different withdrawal scenarios of a rat that can occur whilst the animal is in the testing apparatus. The first example shows a proper withdrawal response in which the animal removes its paw from the heat and immediately licks its paw as a reflex. The second example shows removal of the paw from the heat source which is not due to the heat but rather is voluntary movement of the paw. In the second example there is no licking of the paw or acknowledgement of the heat and should therefore not be included as a proper withdrawal response.