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. Author manuscript; available in PMC: 2017 Dec 12.
Published in final edited form as: Oncogene. 2017 Jun 12;36(42):5819–5828. doi: 10.1038/onc.2017.187

Figure 5.

Figure 5

RNF20 depletion alleviates the inhibitory effect of ATXN7L3 depletion on cell migration. A) MCF-10A cells were transfected with each of the indicated siRNA oligos (12.5 nM final) or a combination thereof; LacZ siRNA was used to bring up the total siRNA concentration to 25 nM in each case. 48 hours later, cells were starved for 7 hours and subsequently collected for Western blot analysis with the indicated antibodies (A). Numbers indicate quantification (Image Lab Software) of the H2Bub1 band, normalized to total H2B. NA=not applicable. Transwell migration assay (B) was performed and analyzed as in Fig, 3A-C. Average migration ±SEM from 5 independent experiments is shown in (C).