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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: Biochim Biophys Acta. 2017 Jul 4;1865(10):1246–1254. doi: 10.1016/j.bbapap.2017.07.001

Figure 2.

Figure 2

The kinetic constants Km and kcat for hydrolysis of FXIII V34X AP (28–41) peptides by WT and mutant thrombins. A) A comparison of the Km values for FXIII V34 AP (yellow), L34 AP (red), F34 AP (purple), W34 AP (light blue), and Y34 AP (green) in the presence of WT thrombin and the single site mutants W60dA, Y60aA, L99A, I174A, W215A, and WE. B) A comparison of the kcat values for FXIII V34 AP (yellow), L34 AP (red), F34 AP (purple), W34 AP (light blue), and Y34 AP (green) in the presence of WT thrombin and the single site mutants W60dA, Y60aA, L99A, I174A, W215A, and WE. The individual kinetic parameters were derived from an HPLC assay described in Materials and Methods. Results represent data from at least three independent trials, and error bars correspond to standard error of the mean.