(A) Immunoblot analysis of Bax and Bcl2 in response to tBHP and mangiferin exposure, in NKE cells. (B) Immunoblot analysis of Caspase 8, tBid, Bax, cytochrome C, Apaf-1, Caspase 9, Caspase 3 and PARP. NKE cells were exposed to tBHP (50 µM) and mangiferin (20 µM) according to the defined protocol. β actin was used as an internal control Control: untreated cells, M: exposed to mangiferin, tBHP: exposed to tBHP for 18 h, M+tBHP: Mangiferin treated for 2 h followed by 18 h of tBHP exposure. For inhibitor studies, M+T+I: cells were treated with LY294002(I) follwed by Mangiferin(M) and Tbhp(T). All data are mean±SD, of 3 independent experiments and were analyzed by one-way ANOVA. “*” represents the significant difference between the normal control and tBHP exposed cells and “#” represents the significant difference between the tBHP exposed and tBHP exposed antioxidant treated cells (P*<0.05, P#<0.05).