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. 2016 Jan 14;5:313–327. doi: 10.1016/j.bbrep.2016.01.011

Fig. 9.

Fig. 9.

(A) Immunoblot analysis of Bax and Bcl2 in response to tBHP and mangiferin exposure, in NKE cells. (B) Immunoblot analysis of Caspase 8, tBid, Bax, cytochrome C, Apaf-1, Caspase 9, Caspase 3 and PARP. NKE cells were exposed to tBHP (50 µM) and mangiferin (20 µM) according to the defined protocol. β actin was used as an internal control Control: untreated cells, M: exposed to mangiferin, tBHP: exposed to tBHP for 18 h, M+tBHP: Mangiferin treated for 2 h followed by 18 h of tBHP exposure. For inhibitor studies, M+T+I: cells were treated with LY294002(I) follwed by Mangiferin(M) and Tbhp(T). All data are mean±SD, of 3 independent experiments and were analyzed by one-way ANOVA. “*” represents the significant difference between the normal control and tBHP exposed cells and “#” represents the significant difference between the tBHP exposed and tBHP exposed antioxidant treated cells (P*<0.05, P#<0.05).