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. 2016 Feb 3;5:401–407. doi: 10.1016/j.bbrep.2016.02.002

Fig. 1.

Fig. 1.

The effect of soluble ST2 on the IL-33 signaling pathway in EL-4 cells that stably express ST2L. (A) Purified ST2-V5 (100 ng) was left untreated or treated with N-glycosidase F and developed on SDS polyacrylamide gel, followed by silver staining. (B) ST2L/EL-4 cells were pretreated with ST2-V5 or In-ST2-V5 (4.1, 20.7, and 103.6 ng/ml, corresponding to 0.11, 0.56, and 2.80 nM, respectively) for 1 h and then stimulated with IL-33 (10 ng/ml, corresponding to 0.56 nM) for 15 min. PBS was added as a control for ST2-V5, In-ST2-V5, and IL-33. Phosphorylation and expression levels of the indicated proteins were detected by western blotting. Results are representative of three independent experiments. p-, phosphorylated.