Fig. 5.

Nck1 is involved in the regulation of Rho GTPases. (A–D) Schwann cell precursors were transfected with Nck1 or control siRNA. Cell lysates were used for an affinity-precipitation assay using GST-CRIB and for an immunoblotting with an anti-Rho GTPase antibody. Data were evaluated using Student's t-test (**, p<0.01; n=3). (E, F) Similarly, an affinity-precipitation assay using GST-RBD was performed. Data were evaluated using Student's t-test (**, p<0.01; n=3).