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. 2016 Mar 24;6:113–123. doi: 10.1016/j.bbrep.2016.03.013

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© 2016 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 6. — The proline-rich region of Dock8 is involved in the interaction with Nck1. (A) Domain organizations (unique proline-rich region [red line], DHR1, and DHR2) in human full-length Dock8 (amino acids 1–2032) were depicted. The positions of proline (P, indicated by red squares) in its proline-rich region (amino acids 181–197) were compared to those in other organisms. The amino acids identical to those in human Dock8 were indicated by red characters. (B) 293T cells were transfected with the plasmids encoding FLAG-tagged Dock8 (the wild type or the mutant harboring P187A, P189A, and P192A) and GST-tagged Nck1. The lysates were used for an immunoprecipitation with an anti-GST antibody and the immunoprecipitates were immunoblotted with anti-FLAG antibody (upper panel). Total GST-Nck1 and FLAG-Dock8 were also shown (middle and lower panels).