Fig. 1.
SDC4 is significantly increased in TGF-β1-induced EMT. A, The images of TGF-β1-induced EMT. Scale bar: 200 μm. A549 cells were exposed in the presence or absence of TGF-β1 (5 ng/ml) for 48 h then images were obtained. B, E-cadherin, N-cadherin and Snail protein expression were determined by Western blot analysis. GAPDH was used as loading control. C, Left: SDC4 mRNA expression was analyzed by real-time RT-PCR using specific primers for SDC4 (Supplementary Table S1). The data were normalized to GAPDH. Each bar represents means ±SEM (n=3). “※” indicates statistically significant (P<0.05). Right: the images of SDC4 expression by immunofluorescent staining. The arrows indicate SDC4 positive cells. Scale bar: 100 μm. D, The effects of SDC4 siRNA on E-cadherin, Vimentin, Snail and Slug expression were determined by Western blot analysis. Cells were seeded in 6-well culture plates at 1×105 cells/well, 48 h after transfection with indicated siRNA(s), then harvest protein samples. GAPDH was used as loading control.