Table 3. Multi-layered mutations were observed in the four study subjects with Gorlin syndrome.

gene	case.1	case.2	case.3	case.4
BCL2				+
BMP2	+	+	+	+
BMP8B	+	+		+
BOC		+	+	+
CDON	+	+	+	+
CSNK1A1L	+	+	+	+
DISP1	+	+		+
DISP2	+	+	+	+
FAT4	+	+	+	+
GLI2	+	+	+
GLI3		+
HHAT	+		+	+
LRP2	+	+	+	+
NPC1	+	+	+	+
MTSS1				+
PTCH2	+		+
PTCHD2	+		+	+
PTCHD3	+	+	+	+
SUFU	+	+	+	+
RUNX2			+
STK36			+	+
WNT9B		+
total number (84)	15	15	16	17

After the validation steps shown in Fig 2, 22 gene alterations were selected from 4 individuals.