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. 2017 Sep 5;11(9):e0005912. doi: 10.1371/journal.pntd.0005912

Fig 1. Expression of S. Typhi proteins and lysteriolysin on recombinant E. coli.

Fig 1

(A) Anti-HisTag antibody revealing positive control protein Annexin3 (A3) as well as lysteriolysin (Hly) and S. Typhi gene encoded proteins (OmpC, FliC, GroEL and SifA). A negative control with pmark (P, stop codons) clones was used instead of the protein. (B) Anti-lyteriolysin antibody revealing Hly gene expression (red box) in all lanes except in the negative control. Expression of S. Typhi proteins and lysteriolysin on recombinant E. coli were detected by Western blot.