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. 2017 Sep 5;11(9):e0005912. doi: 10.1371/journal.pntd.0005912

Fig 4. Antigen presentation of S. Typhi proteins by targets infected with recombinant E. coli.

Fig 4

Ex vivo PBMC from a volunteer collected before (day 0) and 42 days after immunization were co-cultured for 16–18 hrs. with autologous B-LCL targets infected at 1:30 MOI with one of the four recombinant E. coli expressing S. Typhi/Hly (Hly/SifA (SifA), Hly/FliC (FliC), Hly/GroEL (GroEL) and Hly/OmpC (OmpC)) or only Hly (control) proteins. After incubation, cells were stained and the ability of the PBMC to express IFN-γ was analyzed by flow cytometry. CD4+ and CD8+ T cells were evaluated. Numbers represent the percentage of positive cells. The data of a representative volunteer are shown.