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. 2017 Sep 5;11(9):e0005913. doi: 10.1371/journal.pntd.0005913

Fig 3. USUV replicates in murine spinal glial cells ex vivo.

Fig 3

Spinal astrocytes and microglia are readily infected by USUV. Mixed cultures were infected with USUV at a MOI of 2 and fixed at 4 dpi. (A) USUV-infected cells labeled with the pan-flavivirus antibody also express GFAP, suggesting an efficient replication in spinal astrocytes. Rows show different pictures of the same experiment. Scale bar 10 μm. (B). USUV-infected cells labeled with the pan-flavivirus antibody express Iba1, suggesting an efficient replication in spinal microglial cells. Scale bar 20 μm.