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. 2017 Sep 5;15(9):e2002887. doi: 10.1371/journal.pbio.2002887

Fig 1. Developmental stages selected for Cap Analysis of Gene Expression (CAGE) study.

Fig 1

(A) Representative images of chicken embryos used for RNA preparation. (B) Temporal distribution of these selected stages (red dots) during chicken embryogenesis. X-axis: 46 Hamburger and Hamilton (HH) stages represented in numerical order. Y-axis: log scale of incubation time needed to reach these stages (based on reported estimates), e.g., Y value of 2.0 = 100 hours. Numerical values for this plot can be found in S1 Data. (C) Principal coordinates analysis (PCoA) of all 16 developmental samples. Four groups, representing early, mid, mid-late, and late developmental phases, can be recognized. (D) PCoA of all samples. Primary cells are clearly grouped away from each other and from developmental samples. Raw data for PCoA can be accessed at http://fantom.gsc.riken.jp/5/suppl/Lizio_et_al_2017/.