Skip to main content
. 2017 Sep 5;13(9):e1006604. doi: 10.1371/journal.ppat.1006604

Fig 6. Comparison of fungal cell propagation and antifungal gene expression in insects.

Fig 6

A. Comparison of fungal hyphal body (HB) numbers in insect hemocoel 48 hrs post injection. Ten insects were bled for each treatment, and five microscopic fields were observed for each insect. Unpaired t-test was conducted to compare the difference level between WT and mutants. ***, represents the difference level at P < 0.001. B. qRT-PCR analysis of Gal (for gallerimycin) gene expression by insects treated with spores of WT and Blys2-related mutants for 36 hrs. *, P < 0.05. C. qRT-PCR analysis of Gal gene expression by insects treated with spores of WT and Blys5-related mutants for 36 hrs. *, P < 0.05. The Slp1 gene of M. oryzae was used to complement the null mutants of Blys2 (ΔBlys2::Slp1) and Blys5 (ΔBlys5::Slp1), respectively. Overexpress of Blys2 in the WT strain of B. bassiana was performed by using two different constitutive promoters, i.e., the GpdA (to obtain the transformant WT::gp-Blys2) and laccase gene (to obtain the transformant WT::lp-Blys2) promoters.