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The FRET melting curves of HTG-21 G-quadruplex in ionic buffer (A), dialysis buffer (B), cell-free system (C), control system (D), and Xenopus laevis egg extracts (E), with or without 0.2 μM or 0.4 μM TMPyP4, SYUIQ-05, SYUIQ-FM05, and ISD-05.
