Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Calculated melting temperatures (Tm) of HTG21 in ionic buffer, dialysis buffer, cell free system and control system.
| Ionic buffera | Dialysis buffer | Cell free systemb | Control systemc | X. laevis egg extract | |
|---|---|---|---|---|---|
| Tm (°C) | 64±0.8 | 66±0.6 | 68±0.9 | 87±0.6 | 72±0.5 |
Representing 10 mM Tris–HCl buffer containing 100 mM K+.
Representing dialysis buffer containing cell extract.
Representing dialysis buffer containing 40% PEG200.
