Table 3.

Calculated inhibition rates in PCR buffer, cell free system and control system. The inhibition rate was calculated by the formula, inhibition rate (%)=(OD_mut–OD_wt)/OD_mut.

	Cell free systema	X. laevis egg extract	Control systemb
Inhibition rate	21.7±4.0%	40.2±3.8%	86.3±5%
	Dialysis buffer	Dialysis buffer Ac	PCR buffer
Inhibition rate	100.0±1.0%	72.2±4.3%	12.3±5%

^aRepresenting dialysis buffer containing cell extract.

^bRepresenting dialysis buffer containing 40% PEG200.

^cRepresenting dialysis buffer containing extra 1.5 mM MgCl₂.