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. 2015 Oct 3;5:439–447. doi: 10.1016/j.bbrep.2015.09.022

Table 4.

Melting temperatures (Tm) of HTG21 in different systems with co-incubation with 0.2 μM or 0.4 μM ligands.

Ionic buffera Dialysis buffer Cell free systemb Control systemc X. laevis egg extract
No drug (μM) 64±0.8 66±0.6 68±0.9 87±0.6 72±0.5
TMPyP4 0.2 78±0.7 82±1.2 81±0.5 86±0.7 74±0.3
0.4 81±0.9 81±0.6 82±0.9 87±0.6 72±0.4
SYUIQ-05 0.2 69±0.7 68±1.0 71±1.2 83±0.1 70±0.5
0.4 70±0.5 70±0.6 73±0.6 83±0.2 68±0.8
SYUIQ-FM05 0.2 71±0.5 71±0.7 72±0.6 83±0.3 69±0.7
0.4 73±0.6 72±0.5 73±0.7 84±0.4 68±0.8
ISD-05 0.2 71±0.1 71±0.2 73±0.4 84±0.3 67±1.0
0.4 74±0.2 73±0.3 75±0.3 83±0.6 68±0.8
a

Representing 10 mM Tris–HCl buffer containing 100 mM K+.

b

Representing dialysis buffer containing cell extract.

c

Representing dialysis buffer containing 40% PEG200.