Table 4.

Melting temperatures (T_m) of HTG21 in different systems with co-incubation with 0.2 μM or 0.4 μM ligands.

		Ionic buffera	Dialysis buffer	Cell free systemb	Control systemc	X. laevis egg extract
No drug	(μM)	64±0.8	66±0.6	68±0.9	87±0.6	72±0.5
TMPyP4	0.2	78±0.7	82±1.2	81±0.5	86±0.7	74±0.3
	0.4	81±0.9	81±0.6	82±0.9	87±0.6	72±0.4
SYUIQ-05	0.2	69±0.7	68±1.0	71±1.2	83±0.1	70±0.5
	0.4	70±0.5	70±0.6	73±0.6	83±0.2	68±0.8
SYUIQ-FM05	0.2	71±0.5	71±0.7	72±0.6	83±0.3	69±0.7
	0.4	73±0.6	72±0.5	73±0.7	84±0.4	68±0.8
ISD-05	0.2	71±0.1	71±0.2	73±0.4	84±0.3	67±1.0
	0.4	74±0.2	73±0.3	75±0.3	83±0.6	68±0.8

^aRepresenting 10 mM Tris–HCl buffer containing 100 mM K⁺.

^bRepresenting dialysis buffer containing cell extract.

^cRepresenting dialysis buffer containing 40% PEG200.