Fig. 3.

CD44 expression induced Ca²⁺ influx uses CRAC channels to regulate proliferation and Ca²⁺ is required for proper cell cycling. (A) Cell proliferation with extracellular calcium, without extracellular calcium, and with Ni²⁺, an inhibitor of CRAC channels under normal extracellular calcium levels. Results are normalized to percent of the vector control cells with calcium. (B) Cell cycle analysis was performed on CD44 expressing cells via propidium iodide staining and flow cytometry after incubation in calcium containing (open bars) or calcium free (closed bars) media for 24 h after synchronization. Asterisks indicate the statistical significance using the 2-tailed Student's t-test (*p<0.05).