Fig. 6.

CD44 increases phosphatase activity in E6.1 Jurkat cells. (A) A phosphatase activity assay specific for PP1, PP2A, calcineurin, and PP2C. Total represents all measured phosphatase activity. Calcium Independent (Calcium Ind.) is the activity of PP1, PP2A, and PP2C. CaN is the activity of just calcineurin. (B) Western blot for total Akt and phosphorylated Akt with cantharidic acid. The Western blot was repeated 3 times with similar results. (C) Proliferation of cells with different concentrations of cantharidic acid over 48 h. Results are normalized to untreated vector control cells. (D) JC-1 staining for mitochondrial depolarization as measured by flow cytometry (left panel) and spectrofluorometry (right panel). Results are expressed as the ratio of red mitochondrial associated dye to green cytosol associated dye. Asterisks indicated statistical significance using the 2-tailed Student's t-test (A) or the ANOVA with Turkey's multiple comparisons test (C) (*p<0.05).