Table 2.
Bacterial strains and plasmids.
| Bacterial Strains/Plasmids | Description | Reference |
|---|---|---|
| Bacterial Strains | ||
| E. coli DH5α | F2 (w80d DlacZ M15) D(lacZYA-argF) U169 hsdR17(r2M) recA1endA1 relA1 deoR12 phoA supE44 thi-1, gyrA96 | [31] |
| E. coli DC10B | Δdcm in the DH10B background, Dam methylation only | [24] |
| S. aureus COL | Clinical isolate, genomic DNA | [32] |
| S. epidermidis TU3298 | Capable of being transformed with and stably maintaining recombinant plasmids. | [25] |
| Plasmids | ||
| pRMC2 | Derivative of the tetracycline-inducible expression vector pALC2073; anhydrotetracycline inducible expression vector, Ampr in E.coli, Chlr in S. aureus. | [33] |
| pRMC2M | pRMC2 vector with cloning site 2 after TetR gene having (Pst1, Sph1, Mfe1,Nhe1 and HindIII) restriction site. | This Study |
| P1GFP-AgrA | AgrA in cloning site 1 with agrP1 promoter driven GFP, cloned in cloning site 2 of pRMC2M. | This Study |
| P2GFP-AgrA | AgrA in cloning site 1 with agrP2 promoter driven GFP, cloned in cloning site 2 of pRMC2M. | This Study |
| P3GFP-AgrA | AgrA in cloning site 1 with agrP3 promoter driven GFP, cloned in cloning site 2 of pRMC2M. | This Study |
| P1GFP-AgrAD59A | AgrAD59A in cloning site 1 with agrP1 promoter driven GFP, cloned in cloning site 2 of pRMC2M. | This Study |
| P2GFP-AgrAD59A | AgrAD59A in cloning site 1 with agrP2 promoter driven GFP, cloned in cloning site 2 of pRMC2M. | This Study |
| P3GFP-AgrAD59A | AgrAD59A in cloning site 1 with agrP3 promoter driven GFP, cloned in cloning site 2 of pRMC2M. | This Study |
| P1GFP-ΔAgrA | agrP1 promoter driven GFP, cloned in cloning site 2 of pRMC2M. | This Study |
| P2GFP-ΔAgrA | agrP2 promoter driven GFP, cloned in cloning site 2 of pRMC2M. | This Study |
| P3GFP-ΔAgrA | agrP3 promoter driven GFP, cloned in cloning site 2 of pRMC2M. | This Study |