Table 3.

Interaction between AgrA and cognate DNA sequences monitored by Surface Plasmon Resonance spectroscopy.

	P1 promoter			P2 promoter			P3 promoter
	ka (X 10+4 M−1 s−1)	kd (X 10−3 s−1)	KD (X 108 M)	ka (X 10+4 M−1s−1)	kd (X 10−3 s−1)	KD (X 10−8 M)	ka (X 10+4 M−1 s−1)	kd (X 10−3 s−1)	KD (X 10−8 M)
AgrA	1.24	2.38	19.2	1.61	1.06	6.59	1.32	2.02	15.3
AgrA~P	1.54	1.53	9.92	2.76	1.11	4.02	7.05	1.47	2.09
AgrAD59A	1.16	1.93	16.6	2.24	1.60	7.14	1.99	2.13	10.7
AgrAD59A~P	1.07	1.33	12.4	1.45	1.11	7.68	1.26	1.33	10.5