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. 2016 Feb 27;6:54–62. doi: 10.1016/j.bbrep.2016.02.014

Fig. 2.

Fig. 2

A: Structural molecular organization of the rat desmin molecule (P48675) (adapted from [24] and desmin sequence recognized by the three desmin antibodies (clones Y66 (Abcam, ab32362), DE-U-10 (Sigma-Aldrich, D1033) and D33 (Dako, M0760) used for western blot. A dashed line is shown for the D33 antibody as no information on the epitope recognized is available. B, C: Western blot analysis of desmin in plasma from rat, mouse and human species with the three desmin antibodies. Plasma was either depleted for albumin and IgG (B) or treated by combinatorial peptide ligand library (CPLL) (C). M: size marker, C: 50 µg of treated plasma, IP: immunoprecipitated treated plasma with 2 µL of DE-U-10 desmin antibody, LV: left ventricle (10 µg), S: supernatant of LV IP (10 µg), Ab: antibody (2 µL). The antibody used for the western blot is indicated on each blot. Arrows indicate the specific desmin band recognized by the antibodies and ns the non-specific bands.