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. 2016 Feb 27;6:54–62. doi: 10.1016/j.bbrep.2016.02.014

Fig. 4.

Fig. 4

Biochemical characterization of desmin. A: Western blot analysis of desmin in LV proteins (50 µg) extracted from sham- and HF-rats in RIPA and Urea/Thiourea buffer. The ponceau red staining gel (left panel) and the western blot images (right panel) with desmin DE-U-10 (upper panel) and Y66 (lower panel) antibodies at short exposure are presented. B: Western blot analysis of desmin in LV proteins separated using Phos-Tag (8%, 50 µmol/L) affinity electrophoresis performed with the DE-U-10 antibody. LV proteins are extracted in RIPA (50 µg of proteins) (upper panel) or Urea/Thiourea (20 µg of proteins) (lower panel) buffer. The western blot images presented are at long exposure (>1000 s) for RIPA extraction and short exposure (12 s) for Urea/Thiourea extraction. Arrows indicate the specific desmin bands recognized. M: size marker.