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. 2016 Apr 16;6:185–189. doi: 10.1016/j.bbrep.2016.04.005

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© 2016 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 2. — Strep-tagged FocA pulls down TdcE. (A) A Coomassie Brilliant Blue-stained SDS-PAGE (10% w/v polyacrylamide) is shown. Lane M, molecular mass markers (Fermentas); lane 1, purified N-terminally Strep-tagged FocA (FocA-N; 1 μg); lane 2, pull-down of a FocA-N and TdcE mixture (10 μg each); lane 3, pull-down of TdcE (10 μg) without FocA-N; lane 4, purified TdcE (1 μg). (B) The same experiment as shown in part A. but after western blot analysis with anti-PflB antiserum. The migration positions of TdcE and FocA-N are indicated on the right side of panel A., while an arrow on the right side of panel B. indicates the migration position of TdcE. The asterisk denotes an unidentified polypeptide that acted as a loading control.