Skip to main content
. 2015 Nov 30;5:27–34. doi: 10.1016/j.bbrep.2015.11.012

Fig. 2.

Fig. 2

Inhibition of Trx system enhances protein S-sulfhydration. A and B, PX12 induces PC and GAPDH S-sulfhydration. HepG2 cells were firstly treated with 5 µM PX12 for 4 h, and then 50 µM NaHS was added for additional 2 h. After that, the cells were collected for biotin switch assay using antibody against PC (A) and GAPDH (B). *p<0.05 versus control. n=4. C and D, DNCB induces PC and GAPDH S-sulfhydration. HepG2 cells were treated with 40 µM DNCB or 30 min and/or 50 µM NaHS for 2 h. After that, the cells were collected for biotin switch assay using antibody against PC (C) and GAPDH (D). *p<0.05 versus control. n=4. E and F, knockdown of Trx1 stimulates PC and GAPDH S-sulfhydration. HEK293 cells were transfected with Trx1-specific siRNA or negative siRNA for 48 h following incubation with 50 µM NaHS for 2 h. After that, the cells were collected for biotin switch assay using antibody against PC (E) and GAPDH (F). *p<0.05 versus control. n=3.