Fig. 3.

Tet1 knockdown in C3H10T1/2 cells downregulated the expression of Col2 and Col10 during chondrocyte differentiation. (a) (b) (c) Tet1 knockdown cells were generated using retroviral shRNA. For a control, a shRNA against LacZ was introduced into the cells. After these cells were differentiated to chondrocytes, RT-qPCR using primers for Tet1 (a), Col2 (b), and Col10 (c) were conducted. Data are presented as means ± standard deriviations (n=3), ratio to shLacZ at day zero samples. **p< 0.01.