FIGURE 3.
Pretreatment with FL3 or FL37 decreases TNFα- or IFNγ-induced expression of nuclear factor kappa B (NFκB) p65 and Cox2 in Caco2-BBE cells. Cells were pretreated with increasing concentrations of FL3 (A) or FL37 (B) for 1 hour, followed by treatment with 10 ng/mL TNFα or 50 ng/mL IFNγ for 16 hours. Total protein was isolated for Western blotting for expression of NFκB p65, Cox2, PHB, and β-actin (loading control).