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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Nat Protoc. 2017 Sep 7;12(10):2097–2109. doi: 10.1038/nprot.2017.089

Figure 2.

Figure 2

Isl1 positive cardiac progenitor cells. A. Isl1-Cre driven expression of Red Fluorescent Protein (RFP) in a mouse embryoid body. B. RFP+ CPCs are purified by FACS. (62,000 live cells were sorted 14,410 of those were Isl1-positive (23.3%)). C. Immunostaining for Isl1 and flow cytometry analysis of fixed human CPCs. Left plot: negative control with only secondary antibody. (2384 cells were analyzed and of those 31 were only Isl1-positive (1.3%)). Right plot: immunostaining with Isl1 primary antibody. (2750 cells were analyzed and of those 1940 were Isl1-positive (70.3%)). Scale bar 50 μm.