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. Author manuscript; available in PMC: 2017 Sep 15.
Published in final edited form as: Cell Rep. 2017 Jun 20;19(12):2477–2489. doi: 10.1016/j.celrep.2017.05.086

Figure 3. The RNA Recognition Motif of Rct1 is Essential for siRNA Biogenesis and Pericentromeric Heterochromatin Silencing.

Figure 3

(A) Schematic representation of rct1 alleles. rct1 FL contains full-length Rct1 with no mutations. rct1ΔIso lacks the first 175 amino acids corresponding to PPIase domain. rct1-rrm includes two amino acid mutations (red) in the RRM,Y287D and F289D, both of which combined were predicted to abolish the RNA-binding ability of Rct1. rct1ΔC has amino acids 333-428 removed.

(B) Small RNA northern blots of pericentromeric dh/dg derived siRNAs. U6 serves as loading control.

(C) Semi-quantitative RT-PCR of dh/dg and otr1R::ura4 transcript levels in rct1 mutant cells. Truncated ura4-DS/E at the endogenous locus and act1 serves as loading controls, -RT omits the reverse transcription step.