KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| rabbit anti-GFP | Life Technologies | Cat#A-11122; RRID:AB_221569 |
| chicken anti-GFP | Abcam | Cat#ab13970; RRID:AB_300798 |
| rabbit anti-IR25a | Ref. 5 | N/A |
| guinea pig anti-IR40a | Gift from R. Benton | N/A |
| mouse anti-nc82 | DSHB | Cat#nc82; RRID:AB_2314865 |
| goat anti-rabbit Alexa 488 | Life Technologies | Cat#A-11070; RRID:AB_2534114 |
| goat anti-chicken Alexa 488 | Life Technologies | Cat#A-11039; RRID:AB_2534096 |
| goat anti-rabbit Alexa 594 | Life Technologies | Cat#A-11037; RRID:AB_2534095 |
| goat anti-guinea pig Alexa 546 | Life Technologies | Cat#A-11074; RRID:AB_2534118 |
| donkey anti-mouse Cy3 | Jackson ImmunoResearch Labs | Cat#715-165-150; RRID:AB_2340813 |
| Bacterial and Virus Strains | ||
| Biological Samples | ||
| Chemicals, Peptides, and Recombinant Proteins | ||
| Rapiclear 1.47 | SunJin Lab | Cat#RC147001 |
| LiCl | Sigma- Aldrich | Cat#L9650 |
| NaCl | Sigma- Aldrich | Cat#S9625 |
| Critical Commercial Assays | ||
| Deposited Data | ||
| Experimental Models: Cell Lines | ||
| Experimental Models: Organisms/Strains | ||
| D. melanogaster w1118 CS | Gift from A. Simon | N/A |
| D. melanogaster: IR40a1 | Gift from R. Benton | N/A |
| D. melanogaster: IR68aMB05565 | Bloomington Drosophila Stock Center | BDSC:26031 Flybase: FBst0026031 |
| D. melanogaster: IR40a-Gal4 | Bloomington Drosophila Stock Center | (BDSC 41727) Flybase: FBst0041727 |
| D. melanogaster: Ir40a-LexA | Gift from R. Benton | N/A |
| D. melanogaster: VT029048-Gal4 | Vienna Drosophila Resource Center | VDRC: 207464 Flybase: FBst0486274 |
| D. melanogaster: Ir93a-LexA | Ref. 9 | N/A |
| D. melanogaster: IR25a-Gal4 | Bloomington Drosophila Stock Center | BDSC:41728 Flybase: FBst0041728 |
| D. melanogaster: 10XUAS-IVS-mCD8::GFP | Bloomington Drosophila Stock Center | BDSC:32186 Flybase: FBst0032186 |
| D. melanogaster: 20XUAS-IVS-GCaMP6m | Bloomington Drosophila Stock Center | BDSC:42748 Flybase: FBst0042748 |
| D. melanogaster: 13XLexAop-IVS-GCaMP6m | Bloomington Drosophila Stock Center | BDSC:44275 Flybase: FBst0044275 |
| D. melanogaster: R22C06-Gal4 | Bloomington Drosophila Stock Center | BDSC:48974 Flybase: FBst0048974 |
| D. melanogaster: R24G07-Gal4 | Bloomington Drosophila Stock Center | BDSC:49095 Flybase: FBst0049095 |
| D. melanogaster: R24G07-LexA | Bloomington Drosophila Stock Center | BDSC:52726 Flybase: FBst0052726 |
| D. melanogaster: UAS-syb:spGFP 1–10, AOP-CD4:spGFP 11 | Bloomington Drosophila Stock Center | BDSC:64314 Flybase: FBst0064314 |
| D. melanogaster: UAS-Kir2.1 | Ref. 12 | N/A |
| Oligonucleotides | ||
| Recombinant DNA | ||
| pENTR (pCR™8/GW/TOPO®) | Thermo Fisher Scientific | Cat#K250020 |
| pBPLexAp65Uw | Ref. 10 | Addgene Plasmid #26231 |
| pJFRC19-13XLexAop2-IVS-myr::GFP | Ref. 10 | Addgene Plasmid #26224 |
| R22C06-LexAp65 (in pBPLexAp65Uw) | This paper | N/A |
| pJFRC19-13XLexAop-TdTomato in (pJFRC19 backbone) | This paper | N/A |
| Software and Algorithms | ||
| MATLAB | The MathWorks, Inc. | http://www.mathworks.com |
| Fiji | http://fiji.sc | |
| Other | ||