. Author manuscript; available in PMC: 2017 Sep 15.

Published in final edited form as: Breast Cancer Res Treat. 2016 Jul 25;159(1):31–39. doi: 10.1007/s10549-016-3917-6

Table 3.

Distribution of Her-2 D-FISH equivocal cases and “low copy positive” cases per the ASCO/CAP 2013 guidelines corresponding to IHC assessment

		HER-2 IHC

		0	1+	2+	3+
A¹	Yes	6(20.0)	14(13.6)	12(13.8)	1(2.9)
	No	24(80.0)	89(86.4)	75(86.2)	34(97.1)
	Total	30(100.0)	103(100.0)	87(100.0)	35(100.0)
	r_s		−0.104
	P value		0.096
B²	Yes	0(0.0)	2(1.9)	6(6.9)	9(25.7)
	No	30(100.0)	101(98.1)	81(93.1)	26(74.3)
	Total	30(100.0)	103(100.0)	87(100.0)	35(100.0)
	r_s		0.270
	P value		<0.001

Equivocal cases by dual-probe FISH according to the 2013 ASCO/CAP guidelines, i.e. cases with the Her-2/CEP17 ratio < 2.0 and the average number of Her-2 signals per cell >= 4 and <6

“low Her-2 copy positive (LCP)”, i.e. cases with the Her-2/CEP17 ratio >= 2.0 and the average number of Her-2 signals per cell >= 4 and <6.

Her-2 D-FISH: Her-2 dual-probe fluorescent in situ hybridization

IHC: Immunohistochemistry