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. Author manuscript; available in PMC: 2017 Sep 15.
Published in final edited form as: J Autoimmun. 2016 Apr 23;70:1–11. doi: 10.1016/j.jaut.2016.04.001

Figure 2. Specificity of circulating GP autoantibodies from the A5 patient to human recombinant NC1 domains of collagen IV tested by indirect ELISA (A) and Western blot (B).

Figure 2

Reactivity of two other GP patients (GP1, GP2) is shown for comparison. The dashed line in A indicates normal human serum threshold determined as mean + 3xSD. Lane M in panel B shows the position of pre-stained protein molecular weight markers.