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. Author manuscript; available in PMC: 2017 Sep 15.
Published in final edited form as: J Autoimmun. 2016 Apr 23;70:1–11. doi: 10.1016/j.jaut.2016.04.001

Figure 3. Epitope mappaing of the α5NC1-specific autoantibodies from the patient A5 using indirect (A) and inhibition (B) ELISA.

Figure 3

GP1 patient with classical anti-GBM disease is shown for comparison (A). In panel B, A5 patient serum was pre-incubated with various concentrations of the α1NC1 (δ), α5NC1 (●) EA-α5 (○), EB-α5 (▼) or the mixture of EA- and EB-α5 chimeras (●, dashed line). Binding to immobilized α5NC1 domain was measured using ELISA, and relative binding is calculated as a percentage of antibody binding to immobilized α5NC1 in the absence of NC1 monomers or chimeras in solution.