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. Author manuscript; available in PMC: 2017 Sep 15.
Published in final edited form as: Theriogenology. 2004 Sep 15;62(6):971–989. doi: 10.1016/j.theriogenology.2003.12.022

Fig. 9.

Fig. 9

Motility during storage at 4 °C for 15 days (mean ± S.D.) after thawing of Xiphophorus couchianus sperm diluted before freezing at volume ratios of semen to extender–cryoprotectant (HBSS–glycerol) of 1:20, and diluted again immediately after thawing at a ratio of sperm suspension to HBSS of 1:3 (open circles) or to HBSS–glycerol of 1:3 (open squares), or without dilution after thawing (filled circles). Suspensions were prepared with 300 mOsm/kg HBSS.