Table 1.
UV exposure of embryos at 2–32 cell stage from wild-type (wild) and transgenic (fli-1) zebrafish
| Fish source | UVB (J/cm2) | UVA (J/cm2) | Number of embryos | Mortality (%) | Hatch (%) | Malformation (%)
|
|
|---|---|---|---|---|---|---|---|
| Total | Severe | ||||||
| Wild | 4.67 | 0 | 40 | 5 ± 7 | 94 ± 8 | 19 ± 1 | 6 ± 0 |
| 4.67 | 211.5 | 40 | 0 ± 0 | 100 ± 0 | 9 ± 12 | 6 ± 8 | |
| Ra | 100 | 100 | 54 | 9 | |||
| 6.22 | 0 | 100 | 23 ± 30 | 65 ± 42 | 55 ± 33 | 47 ± 34 | |
| 6.22 | 126.9 | 95 | 23 ± 29 | 58 ± 43 | 75 ± 34 | 60 ± 32 | |
| R | 3 | 20 | 0 | 0 | |||
| fli-1 | 4.67 | 0 | 60 | 5 ± 9 | 93 ± 12 | 7 ± 6 | 2 ± 3 |
| 4.67 | 211.5 | 60 | 30 ± 22 | 60 ± 33 | 5 ± 5 | 2 ± 4 | |
| R | 0 | 0 | 23 | 0 | |||
| 6.22 | 0 | 170 | 20 ± 29 | 72 ± 29 | 35 ± 37 | 18 ± 19 | |
| 6.22 | 126.9 | 129 | 23 ± 29 | 69 ± 38 | 33 ± 35 | 32 ± 34 | |
| R | 0 | 0 | 6 | 0 | |||
Data were normalized with the non-exposed controls, and presented as mean ± SD.
a
Percentage of recovery (R) was calculated based on the formula: R = (t1 − t2)/t1 × 100 for mortality and malformation, and R = (t2 − t1)/(1 − t1) × 100 for hatch where t1 are means of UVB treatment only, and t2 are means for treatment after UVA photo recovery; R was considered to be 0 when negative values occurred..