Skip to main content
. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: J Mol Cell Cardiol. 2017 Jul 25;111:69–80. doi: 10.1016/j.yjmcc.2017.07.010

Figure 4. Inhibiting Cx45CT dimerization alters Cx45 cellular localization.

Figure 4

Representative immunofluorescence images (red) of methanol fixed cells stably expressing Cx45 WT (left) or 6E (right). Co-immunostaining (green) for either E-Cadherin (MDCK) or β-catenin (Hek-293T) was used to mark the plasma membranes of the fixed cells (blue = DAPI). A) Clonally selected MDCK cells stably expressing mouse Cx45 WT or 6E. B) Bulk selected MDCK cells stably expressing human Cx45 WT or 6E. C) Bulk selected Hek-293T cells stably expressing mouse Cx45 WT and 6E. D) Isolated time-lapse frames of clonally selected MDCK cells expressing C-terminal eGFP fusions of mouse Cx45 WT or 6E (green). White arrows in the insets indicate gap junction plaques. Scale bar A–C = 20 μm and D = 10 μm.