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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: J Mol Cell Cardiol. 2017 Jul 25;111:69–80. doi: 10.1016/j.yjmcc.2017.07.010

Figure 7. Inhibiting Cx45CT dimerization alters phosphorylation and protein partner interactions.

Figure 7

MDCK cells stably expressing Cx45 WT or 6E were cultured, lysed, and immunoprecipitated to assess total phosphorylation levels and interactions with β-tubulin, Drebrin, and Nedd4. A) Representative Western blots of immunoprecipitated Cx45 WT or 6E blotting with a general anti-pY antibody. (n=3, ** P<0.01) Representative Western blot of B) β-tubulin (n=7, ***P<0.001), C) Drebrin (n=3, **P<0.01), and D) Nedd4 (n=3, **P<0.01) co-immunoprecipitated with either Cx45 WT or 6E. Data presented as mean fold change + s.e.m. relative to the first WT replicate. Statistics are two-tailed unpaired Student’s T-test.