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. Author manuscript; available in PMC: 2018 Sep 15.
Published in final edited form as: ACS Chem Biol. 2017 Aug 15;12(9):2260–2263. doi: 10.1021/acschembio.7b00521

Figure 2.

Figure 2

Contribution of the N–terminal extension of hUNG2 to steady–state turnover in the absence and presence of 20% PEG8K. (a) Substrate concentration vs velocity plots for hUNG2 and hUNGcat using U19FAM DNA in the absence of 20% PEG8K. (b) Substrate concentration vs velocity plots for hUNG2 and hUNGcat using U19FAM in the presence of 20% PEG8K. (c) [Substrate] vs velocity plot using U90FAM DNA in the absence of PEG8K, and (d) in the presence of 20% PEG8K. All rate measurements were performed in triplicate and the mean and standard deviations are indicated.