Table 2. The thirteen of the nineteen monoallelically methylated and expressed genes that returned informative blast hits.

Gene	Accession	Expressed allele	Function
yippee-like 1	LOC100642754	MeDIP	Yippee is an intracellular protein with a zinc-finger like domain. DNA methylation of a CpG island near the yippie-like 3 promoter in humans represents a possible epigenetic mechanism leading to decreased gene expression in tumours (Kelley et al., 2010).
toll-like receptor Tollo	LOC100644648	MeDIP	Tollo regulates antimicrobial response in the insect respiratory epithelium (Akhouayri et al., 2011).
zinc finger protein Elbow	LOC100650465	MeDIP	The elbow (elB) gene is involved in the formation of the insect tracheal system (Dorfman et al., 2002).
heterogeneous nuclear ribonucleoprotein A3	LOC100651168	MeDIP	Heterogeneous nuclear ribonucleoproteins associated with precursors of functional, protein coding mRNAs (Dreyfuss et al., 1993).
calmodulin-lysine N-methyltransferase-like	LOC100749522	MRE	Calmodulin-lysine N-methyltransferase catalyses the trimethylation of a lysine residue of calmodulin. Calmodulin is a ubiquitous, calcium-dependent, eukaryotic signalling protein with a large number of interactors. The methylation state of calmodulin causes phenotypic changes in growth and developmental processes (Magnani et al., 2010).
Na/K/Ca exchanger CG1090	LOC107998466	MRE	CG1090 functions in the maintenance of calcium homeostasis.
Shaker	LOC100648438	MeDIP	Shaker is involved in the operation of potassium ion channel. Shaker expression was upregulated in sterile versus reproductive honeybee workers (Cardoen et al., 2011).
Centrosomal and chromosomal factor-like	LOC105665737	MeDIP	Essential protein required for proper condensation of mitotic chromosomes and progression through mitosis. Expressed during oogenesis in Drosophila (Kodjabachian et al., 1998).
excitatory amino acid transporter 1	LOC100744217	MRE	Excitatory amino acid transporters are neurotransmitter transporters. Excitatory amino acid transporter 3 expression was upregulated in sterile honeybee workers (Cardoen et al., 2011). Excitatory amino acid transporter 1 expression differences were also associated with worker - queen differentiation in the paper wasp Polistes metricus (Toth et al., 2014).
aminopeptidase M1-like	LOC105666993	MeDIP	M1 aminopeptidases are zinc-dependent enzymes that catalyze the removal of amino acids from the N terminus of polypeptides (Drinkwater et al., 2017).
ras GTPase-activating protein nGAP-like	LOC100652225	MeDIP	Ras GTPase-activating protein 1 was found to be upregulated in reproductive honeybee workers (Cardoen et al., 2011). It is involved in oocyte meiosis.
neuromedin-B receptor-like	LOC100745453	MeDIP	In humans, this G protein-coupled receptor binds neuromedin B, a peptide that stimulates mitosis in gastrointestinal epithelial tissue.
bicaudal D-related protein homolog	LOC100650109	MeDIP	Bicaudal is involved in embryonic pattern formation in Drosophila (Markesich et al., 2000). It is thought to be involved in the differentiation between soldiers and workers in the termite Reticulitermes flavipes (Scharf et al., 2003). Bicaudal protein D has been shown to be methylated more in eggs than sperm in honeybees (Drewell et al., 2014).