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. Author manuscript; available in PMC: 2018 May 30.
Published in final edited form as: Biochemistry. 2017 May 18;56(21):2637–2640. doi: 10.1021/acs.biochem.7b00228

Figure 3.

Figure 3

Trp252 sidechain is solvent-exposed. (A) Superposition of the apo (1UEJ, backbone:purple, sidechains:orange; only folded core shown) and phorbol 13-acetate-complexed (1PTR, backbone:silver, sidechains/ligand:gray) structures of C1Bδ. (B) Expansion of the loop region showing two ordered water molecules that take place of the phorbol ester oxygens in the apo C1Bδ. (C) CLEANEX-PM buildup curve for the Hε1 of the Trp252 sidechain. The results of two independent experiments are shown with open circles. Solid line is the fit to Eq. S9.