Skip to main content
. Author manuscript; available in PMC: 2018 Mar 15.
Published in final edited form as: Clin Cancer Res. 2017 Jun 19;23(18):5446–5459. doi: 10.1158/1078-0432.CCR-17-0342

Figure 4.

Figure 4

High score PSF promotes tumor growth, local invasion, and visceral metastasis in the mammary fat pads of nude mice. A) Graph of tumor volume of xenografts of MDA-MB-231 cells were injected in mammary fat pads of nude mice and treated with low or high score PSF in the absence of collagen (Mean ± SEM). B) Tumor volumes at week 8 of experiment described in A. (p = 0.0167; High, n = 3; Low, n = 7; Mann Whitney test). C) H&E of representative xenograft from the low score PSF group from experiment in A (scale bar = 100μm). D) H&E of representative xenograft from the high score PSF group (scale bar = 100μm). E) Graph of tumor volume of xenografts of MDA-MB-231 cells injected in mammary fat pads of nude mice and treated with low or high score PSF in presence of collagen (Mean ± SEM) (6 weeks, p = 0.0006; 7 weeks, p = 0.0401; Low, n = 8; High, n = 7; Mann Whitney test). F) Tumor volumes at week 7 of experiment described in E (Mean ± SEM) (p = 0.0229; High, n = 7; Low, n = 9, Mann Whitney Test). G) H&E of representative xenograft from the low score PSF group from experiment in E (scale bar = 100μm). H) Image of a representative peritoneum of mouse treated with low and high score PSF (Metastasis observed in 0/5 mice treated with low score PSF and 2/5 mice treated with high score PSF) I) H&E of a representative peritoneum of mouse treated with low and high score PSF (scale bar = 100μm).