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. Author manuscript; available in PMC: 2018 Mar 15.
Published in final edited form as: Clin Cancer Res. 2017 Jun 19;23(18):5446–5459. doi: 10.1158/1078-0432.CCR-17-0342

Figure 6.

Figure 6

Invasion inducing capacity of low and high score PSF is maintained across cell lines and correlates with reduction in IGFBP. A) Invasion assays were performed on the indicated 4 cell lines using low and high score fluids from three different patients in each case (scale bar = 100μm). B) Cytokine array exposed to high and low score PSF. The red boxes indicate the positions of IGFBP 1, 2, 3,and 4 on the array. C) Quantification of IGFBPs from cytokine array normalized to positive control (Mean ± SEM) 8 different patient samples were used for analysis (High, n = 4; Low, n = 4). D) Western blot analysis of IGFBP-5 in mammary glands of nude mice treated with different low and high score PSF. Actin was used as a loading control. (n = 6 mice treated separately; High, n = 3; Low, n = 3). E, F) Model of the mode of action of low and high score PSF, respectively. See text for details.