Table 1. Tricarbonic acid cycle and glycolysis metabolite level alteration in Nf2-deficient cells.

Levels of metabolites as measured by gas chromatography and high performance liquid chromatography. Bolded, underlined numbers indicate significant differences (p ≤ 0.05) between the groups shown, metabolite ratio of <1.00. Greyed, underlined boxes indicate significant difference (p ≤ 0.05) between the groups shown; metabolite ratio of ≥ 1.00. Two-tailed Welch’s t-test was used to compare FH912 and FC912 cells, n = 5. Two-way ANOVA contrasts were used to compare Nf2^−/− and Nf2^f/f MEFs and to evaluate cerulenin treatment effects.

Super Pathway	Sub Pathway	Biochemical Name	Fold change
			Two-Way ANOVA Contrasts	Welch’s Two-Sample t-Test
			$\frac{\text{MEF}Nf{\mathit{2}}^{-/-}}{\text{MEF}Nf{\mathit{2}}^{f/f}}$	$\frac{\text{FH}912}{\text{FC}912}$
Energy	TCA Cycle	citrate	1.47	0.45
		succinate	2.62	1.56
		fumarate	1.23	0.47
		malate	1.94	0.33
	Oxidative Phosphorylation	acetylphosphate	1.8	0.45
		pyrophosphate	0.81	0.78
		phosphate	1.01	0.74
	Glycolysis, Gluconeogenesis, and Pyruvate Metabolism	glucose	0.32	1.45
		glucose-6-phosphate	0.74	0.05
		glucose 1-phosphate	1.12	0.68
		fructose-6-phosphate	0.82	0.16
		3-phosphoglycerate	0.12	0.52
		pyruvate	0.56	1.59
		lactate	1.13	1.1
		glycerate	0.35	0.89