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. Author manuscript; available in PMC: 2018 Sep 15.
Published in final edited form as: Cancer Res. 2017 Jul 20;77(18):4934–4946. doi: 10.1158/0008-5472.CAN-16-3541

Figure 7.

Figure 7

P4 decreases pre- and mature tRNA levels in ER+PR+ PDX breast tumors. A and B, qPCR analysis of primary tRNA levels in UCD4 (A) and UCD65 (B) tumors grown in mice supplemented with E2, E2+P4, or E2+MPA (UCD4 only). Three independent biological replicates were used for analysis. Bars represent mean ± SEM. Significance was determined using one-way ANOVA followed by Tukey post-hoc multiple comparison tests (A) or Students t-test (B). C, Northern blot analysis of mature tRNA families (i.e. all Arg-TAT species) in UCD 4 tumors grown in mice supplemented with E2 or E2+P4. Three independent biological replicates were used. Quantified was normalized to RNA Pol I-regulated 5.8S rRNA. Quantification is graphed on the right as mean ± SEM. Significance was determined using a Student’s t-test for each tRNA group.